Molecular Characterization of Saccharomyces cereviceae using RAPD Molecular Markers

Yeasts are eukaryotic microorganisms including 1,500 species. They are an important model organism in modern cell biology research, and are one of the most thoroughly researched eukaryotic microorganisms. Some yeast can find potential application in the field of bioremediation. In the present investigation the yeast samples were isolated on YPD medium from natural sources like ripened fruits, molasses, milk and milk products. They were identified using standard microbiological techniques. The DNA from the yeast cells was isolated by phenol:chloroform method and the diversity of the yeast samples were determined by Random amplified polymorphism using OPD18, 19 and 20 at an annealing temperature of 35C. The amplification of the DNA showed multiple banding patterns with different molecular weight and the banding patterns of all the four yeast isolate had different banding pattern and they were identified to be of different strains of the yeast. With primer OPD18, isolate 1 showed only four prominent bands and the other three isolates showed banding pattern with a minimum of 8 prominent bands. The isolates with OPD 19 primer amplified with more than 12 bands except in the isolate 1 which showed only 3 prominent band. The amplified product size was mainly found to be between 2001000 kilo Daltons. The amplification was carried out with OPD 20 primer and the isolates showed varied banding patterns. The amplified product length ranged from 3002500 kilo Daltons. The isolate 3 and 4 had very few common bands with the same product length.